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KSArabido.wps
Research Proposal for Aspects of Arabidopsis thaliana
Compiled by Jeannie MacAdams for The Paper Store, November, 1999
Introduction
The mouse ear
cress Arabidopsis thaliana is a member of the Brassicaeae that
lends itself well to genetic study in that it has only a small quantity
of DNA. The plant itself has little commercial value aside from that as
a research tool, and it has no aesthetic horticultural value at all. It
is a small plant that can be grown by the hundreds in cells on a
laboratory bench, and its small quantity of DNA makes it useful in
identifying genes of other organisms (Wheeler, 1994).
Normal flower
color varies between solid white petals to green petals only tinged with
white. Manipulation of flower color is of interest not because of any
potential ornamental value, but because of the mechanisms of gene
expression in the visible appearance of the yellow pigment anthocyanin.
In like manner,
information regarding cold tolerance and the biochemical changes that
occur within the plant in response to cold are not of interest primarily
for culture of the plant itself except as it applies to cultural
conditions that need to be maintained for optimum life cycle completion
time. Rather, Arabidopsis’ response to cold stress is of interest
because facts learned from it can be applied to investigations in human
systems (Stockinger, Gilmour and Thomashow, 1997). This investigation
seeks to determine if enough anthocyanin can be concentrated in petal
cells so that they express a visible yellow color, and if cold stress
has any effect on visible levels of anthocyanin concentrations in petal
cells.
Literature Review
Arabidopsis
naturally contains anthocyanin, and Lloyd, Walbot and Davis (1992) were
able to cause anthocyanin "pathway-specific transcriptional activators R
and C1 from the monocot maize were expressed in two dicots,
Arabidopsis thaliana and Nicotiana tabacum. Expression of R
caused augmented anthocyanin pigmentation in both plant species and
augmented trichome (hair) production in Arabidopsis" (p. 1773),
but C1 had no effect alone. Expression of both in Arabidopsis
resulted in expression of anthocyanins in tissues that normally contain
none, such as root, petal and stamen tissues. In more recent research,
Walker, Davison, Bolognesi-Winfield, James, Srinivasean, Blundell, Esch,
Marks and Gray (1999), the researchers isolated by positional cloning
the transparent testa glabra1 (TTG1) locus they had previously
determined as regulating development of anthocyanins in Arabidopsis.

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